Synthesis of [12]aneN3-dipeptide conjugates as metal-free DNA nucleases

Authors: Li, Zhi-Fen; Chen, Hua-Long; Zhang, Li-Jun; Lu, Zhong-Lin; Bioorganic & Medicinal Chemistry Letters; (2012); 10.1016/j.bmcl.2012.01.075

In this Letter, a series of macrocyclic polyamine [12]aneN(3)-dipeptide conjugates as a new type of metal-free nucleases were synthesized and fully characterized with (1)H NMR, (13)C NMR, IR, and HR-MS. Results indicate that these conjugates can bind to calf thymus DNA mainly through electrostatic interaction and can cleave the plasmid DNA at 200 ?M (pH 7.2, 37°C), with an acceleration of 10(6)-fold via hydrolytic pathway.